Journal: bioRxiv

Article Title: Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptor

doi: 10.1101/2021.07.02.450968

Figure Lengend Snippet: GRE-transcriptional activity of GR in the absence or presence of FABP1, FABP2, FABP3, FABP4 or FABP5 in response to vehicle control or increasing concentrations of (A) hydrocortisone (B) dexamethasone or (C) prednisolone, was assessed using a reporter gene in COS-7 cells after 24 h treatment (n=3). (D) Expression of the GR in naïve COS-7 cells or following transfection with GR determined by immunoblotting. Transcriptional activity of the endogenous or transfected GR in the presence of FABP4 in response to (E) dexamethasone or (F) prednisolone (n=3). (G) FABP translocation to the nucleus in COS-7 cells transfected with GR and GFP-FABP1 or GFP-FABP4 following 24 h treatment with vehicle or an EC 80 concentration of hydrocortisone, dexamethasone or prednisolone (n=3). Data are mean ± SEM from n independent experiments, as stated. For concentration-response curves, symbols show means and error bars, S.E.M. *** p<0.001, two-way ANOVA with Sidak’s multiple comparison test. For bar graphs, bars show the mean, error bars the S.E.M. and symbols show the independent data points for each experiment. *** p<0.001, two-way ANOVA with Dunnett’s multiple comparisons test.

Article Snippet: Immunoblotting used primary antibodies recognising FABP1 (Abcam (AB76812; rabbit, 1:300), FABP2 (gift from Dr Satoshi Kaiura, Dainippon Sumi-tomo Pharma Co. Ltd., Osaka, Japan; mouse; 1:400), FABP3 (R&D Systems MAB1678; rabbit, 1:200), FABP4 (Abcam AB92501; rabbit, 1:1000), FABP5 (Abcam AB37267; rabbit, 1:200), GR (Abcam AB183127; rabbit, 1:400) or β-actin (Cell Signaling Technology 3700S; mouse, 1:5000).

Techniques: Activity Assay, Control, Expressing, Transfection, Western Blot, Translocation Assay, Concentration Assay, Comparison

Journal: Genes & Nutrition

Article Title: Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs

doi: 10.1186/s12263-019-0643-9

Figure Lengend Snippet: Primer sequences

Article Snippet: All primary antibodies were raised in rabbits and were suitable for detecting specific porcine proteins: DECR1(sc-393473, Santa Cruz Biotechnology, Dallas, TX), FABP3 (orb156814, Biorbyt, Berkeley, CA), HSPA1L (bs-18079R, Bioss, Columbia, TN), LPIN1 (sc-50050, Santa Cruz), PEKM (sc-31712, Santa Cruz), TNNI1 (orb106871, Biorbyt), TNNT3 (BWB-MS201C, Genway Biotech, San Diego, CA), PPARGC-1 (bs-7535R, Bioss), MSTN (ab98337, Abcam), FASN (ab128856, Abcam), PCK1 (ab87340, Abcam), and GAPDH (sc-20357, Santa Cruz).

Techniques: Sequencing

Journal: Genes & Nutrition

Article Title: Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs

doi: 10.1186/s12263-019-0643-9

Figure Lengend Snippet: Differentially expressed genes related to energy metabolism, muscle structure, and stress response

Article Snippet: All primary antibodies were raised in rabbits and were suitable for detecting specific porcine proteins: DECR1(sc-393473, Santa Cruz Biotechnology, Dallas, TX), FABP3 (orb156814, Biorbyt, Berkeley, CA), HSPA1L (bs-18079R, Bioss, Columbia, TN), LPIN1 (sc-50050, Santa Cruz), PEKM (sc-31712, Santa Cruz), TNNI1 (orb106871, Biorbyt), TNNT3 (BWB-MS201C, Genway Biotech, San Diego, CA), PPARGC-1 (bs-7535R, Bioss), MSTN (ab98337, Abcam), FASN (ab128856, Abcam), PCK1 (ab87340, Abcam), and GAPDH (sc-20357, Santa Cruz).

Techniques: Binding Assay

Journal: Genes & Nutrition

Article Title: Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs

doi: 10.1186/s12263-019-0643-9

Figure Lengend Snippet: Significant KEGG pathway analysis

Article Snippet: All primary antibodies were raised in rabbits and were suitable for detecting specific porcine proteins: DECR1(sc-393473, Santa Cruz Biotechnology, Dallas, TX), FABP3 (orb156814, Biorbyt, Berkeley, CA), HSPA1L (bs-18079R, Bioss, Columbia, TN), LPIN1 (sc-50050, Santa Cruz), PEKM (sc-31712, Santa Cruz), TNNI1 (orb106871, Biorbyt), TNNT3 (BWB-MS201C, Genway Biotech, San Diego, CA), PPARGC-1 (bs-7535R, Bioss), MSTN (ab98337, Abcam), FASN (ab128856, Abcam), PCK1 (ab87340, Abcam), and GAPDH (sc-20357, Santa Cruz).

Techniques: Migration, Infection, Starch

Journal: Genes & Nutrition

Article Title: Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs

doi: 10.1186/s12263-019-0643-9

Figure Lengend Snippet: Western blots for a selection of differentially expressed proteins. Targeted protein DECR1, FABP3, HSPA1L, LPIN1, PFKM, TNNT3, TNNI1, PPARGC-1, MSTN, FASN, and PCK1, with GAPDH as a reference protein. a Representative sample from each treatment fractionated by SDS-PAGE, blotted and then detected with specific antibodies. b Semi-quantification of protein content from scans of band, ( n = 4 pools of two pigs per treatment)

Article Snippet: All primary antibodies were raised in rabbits and were suitable for detecting specific porcine proteins: DECR1(sc-393473, Santa Cruz Biotechnology, Dallas, TX), FABP3 (orb156814, Biorbyt, Berkeley, CA), HSPA1L (bs-18079R, Bioss, Columbia, TN), LPIN1 (sc-50050, Santa Cruz), PEKM (sc-31712, Santa Cruz), TNNI1 (orb106871, Biorbyt), TNNT3 (BWB-MS201C, Genway Biotech, San Diego, CA), PPARGC-1 (bs-7535R, Bioss), MSTN (ab98337, Abcam), FASN (ab128856, Abcam), PCK1 (ab87340, Abcam), and GAPDH (sc-20357, Santa Cruz).

Techniques: Western Blot, Selection, SDS Page

Journal: Cell Biochemistry and Biophysics

Article Title: Fabp3 Inhibits Proliferation and Promotes Apoptosis of Embryonic Myocardial Cells

doi: 10.1007/s12013-010-9148-2

Figure Lengend Snippet: Primer sequences and annealing temperature used in PCR

Article Snippet: Protein concentration determinations using a protein assay reagent kit, and western blotting using a monoclonal rabbit anti-cTnT antibody (CHEMICON, USA), anti-FABP3 antibody (Genetex, USA), and a monoclonal rabbit anti-GAPDH antibody (Proteintech Group, Inc., China), were performed as described previously [ ].

Techniques:

Journal: Cell Biochemistry and Biophysics

Article Title: Fabp3 Inhibits Proliferation and Promotes Apoptosis of Embryonic Myocardial Cells

doi: 10.1007/s12013-010-9148-2

Figure Lengend Snippet: The changes in FABP3 mRNA expression during differentiation. a The changes in FABP3 mRNA expression during differentiation were confirmed by RT-PCR. b Western blotting shows the changes in FABP3 protein expression during differentiation. The results represent the mean ± SD of six experiments

Article Snippet: Protein concentration determinations using a protein assay reagent kit, and western blotting using a monoclonal rabbit anti-cTnT antibody (CHEMICON, USA), anti-FABP3 antibody (Genetex, USA), and a monoclonal rabbit anti-GAPDH antibody (Proteintech Group, Inc., China), were performed as described previously [ ].

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Cell Biochemistry and Biophysics

Article Title: Fabp3 Inhibits Proliferation and Promotes Apoptosis of Embryonic Myocardial Cells

doi: 10.1007/s12013-010-9148-2

Figure Lengend Snippet: Establishment of stable overexpressing FABP3 P19 cells. Western blot analysis of FABP3 expression. P19 cells were transfected with pcDNA3.1Myc/HisB-FABP3 or empty vector. Total cellular protein was extracted from the cells and subjected to western blot analysis. The experiment was repeated three times with similar results

Article Snippet: Protein concentration determinations using a protein assay reagent kit, and western blotting using a monoclonal rabbit anti-cTnT antibody (CHEMICON, USA), anti-FABP3 antibody (Genetex, USA), and a monoclonal rabbit anti-GAPDH antibody (Proteintech Group, Inc., China), were performed as described previously [ ].

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation

Journal: Cell Biochemistry and Biophysics

Article Title: Fabp3 Inhibits Proliferation and Promotes Apoptosis of Embryonic Myocardial Cells

doi: 10.1007/s12013-010-9148-2

Figure Lengend Snippet: Effect of FABP3 on cell differentiation. a There was no significant difference in the morphology of P19 cell lines overexpressing the FABP3 gene on days 0, 4, and 12 of differentiation and the control cells. b Expression of cardiac muscle-specific molecular markers (cTnT, alpha-MHC, GATA4, and MEF2c) was analyzed by quantitative real-time RT-PCR at various time points during the stimulation of differentiation (days 6, 8, and 12). The results represent the mean ± SD of six experiments (* P < 0.05, ** P < 0.001)

Article Snippet: Protein concentration determinations using a protein assay reagent kit, and western blotting using a monoclonal rabbit anti-cTnT antibody (CHEMICON, USA), anti-FABP3 antibody (Genetex, USA), and a monoclonal rabbit anti-GAPDH antibody (Proteintech Group, Inc., China), were performed as described previously [ ].

Techniques: Cell Differentiation, Control, Expressing, Quantitative RT-PCR

Journal: Cell Biochemistry and Biophysics

Article Title: Fabp3 Inhibits Proliferation and Promotes Apoptosis of Embryonic Myocardial Cells

doi: 10.1007/s12013-010-9148-2

Figure Lengend Snippet: Effect of FABP3 on cell proliferation. a The effects of FABP3 on cell proliferation were detected by MTT assays. b Cell cycle profile of FABP3 transfectants. The results represent the mean ± SD of six experiments (* P < 0.05, ** P < 0.001)

Article Snippet: Protein concentration determinations using a protein assay reagent kit, and western blotting using a monoclonal rabbit anti-cTnT antibody (CHEMICON, USA), anti-FABP3 antibody (Genetex, USA), and a monoclonal rabbit anti-GAPDH antibody (Proteintech Group, Inc., China), were performed as described previously [ ].

Techniques:

Journal: Cell Biochemistry and Biophysics

Article Title: Fabp3 Inhibits Proliferation and Promotes Apoptosis of Embryonic Myocardial Cells

doi: 10.1007/s12013-010-9148-2

Figure Lengend Snippet: Effect of FABP3 on cell apoptosis. Apoptosis was assayed by the binding of annexin V-FITC. FABP3 can promote apoptosis induced by serum deprivation in P19 cells. The results represent the mean ± SD of six experiments (* P < 0.05)

Article Snippet: Protein concentration determinations using a protein assay reagent kit, and western blotting using a monoclonal rabbit anti-cTnT antibody (CHEMICON, USA), anti-FABP3 antibody (Genetex, USA), and a monoclonal rabbit anti-GAPDH antibody (Proteintech Group, Inc., China), were performed as described previously [ ].

Techniques: Binding Assay